Analogs of Eap Protein Are Conserved and Prevalent in Clinical Staphylococcus aureus Isolates
Open Access
- 1 November 2001
- journal article
- research article
- Published by American Society for Microbiology in Clinical and Diagnostic Laboratory Immunology
- Vol. 8 (6) , 1271-1276
- https://doi.org/10.1128/cdli.8.6.1271-1276.2001
Abstract
Map and Eap are secreted Staphylococcus aureus proteins that interact with various extracellular matrix molecules. PCR analysis using map primers yielded positive reactions in 97.9% of S. aureus isolates but not in Staphylococcus epidermidis isolates. Cloning and sequencing of the conferring genes revealed a high degree of overall homology combined with size variability of the gene product due to various repeat numbers and early translation termination in a poly(A) region. Thus, Map and Eap may provide a potential novel tool for S. aureus identification and typing.Keywords
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