Evaluation of DNA Fragment Sizing and Quantification by the Agilent 2100 Bioanalyzer

Abstract
Capillary electrophoresis (CE) achieves efficient separation of molecular species by the application of high voltages to samples in solution ( 1). Commercial CE units, available for slightly more than a decade, have found numerous applications ( 2)( 3)( 4)( 5)( 6), but are expensive (∼$60 000) and require substantial user training and experience. Recent advances have allowed CE to be performed on microchip devices ( 7)( 8)( 9)( 10)( 11). We evaluated the Agilent 2100 Bioanalyzer (Agilent Technologies), which represents a new generation of CE instruments that use this technology.