Role of phospholipids in the binding activity of vasoactive intestinal peptide receptors

1 May 1985

journal article
research article
Published by Springer Nature in Cellular and Molecular Life Sciences

Vol. 41 (5) , 631-633
https://doi.org/10.1007/bf02007693

Abstract

Phospholipase digestion of rat intestinal epithelial cell membranes was performed in order to study the influence of membrane phospholipids on the binding activity of VIP receptors. Phospholipases A₂ and C strougly (ED₅₀≅4×10⁻² and 4×10⁻¹ μg/ml, respectively) and rapidly reduced¹²⁵I-VIP binding to membranes whereas phospholipase D was ineffective. This suggests an important role of both hydrophobic and hydrophilic groups of phospholipids on VIP receptor binding activity.

Keywords

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