Effect of AD6 (8-monochloro-3-beta-diethylamino-ethyl-4-methyl-7-ethoxycarbonylmethoxy coumarin) on cyclic nucleotide phosphodiesterases in human platelets
- 1 March 1988
- journal article
- research article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 44 (3) , 226-228
- https://doi.org/10.1007/bf01941715
Abstract
The effect of AD6 (8-monochloro-3-beta-diethylamino-ethyl-4-methyl-7-ethoxycarbonylmethoxy coumarin), an inhibitor of platelet aggregation, on cyclic nucleotide metabolism was investigated. AD6 inhibited selectively human platelet cyclic GMP phosphodiesterase, which was separated from cyclic AMP phosphodiesterase by DEAE-cellulose chromatography. Addition of AD6 to washed platelets increased cyclic GMP levels significantly in two minutes. These results could be useful in elucidating the action of AD6 on intact platelets.Keywords
This publication has 15 references indexed in Scilit:
- AD6(8-monochloro-3-beta-diethylamino-ethyl-4-methyl-7-ethoxycarbonyl-methoxy coumarin) Inhibits the release of arachidonic acid in human platelets stimulated by thrombinThrombosis Research, 1987
- The effect of AD6 (8-monochloro-3-β-diethylamino-ethyl-4-methyl-7-ethoxycarbonylmethoxycoumarin) on washed human platelet aggregation induced by platelet activating factor (PAF) and epinephrineEuropean Journal of Pharmacology, 1986
- Action of AD6 (8-monochloro-3-beta-diethylaminoethyl-4-methyl-7-ethoxycarbonylmethoxy coumarin) on human platelets in vitroNaunyn-Schmiedebergs Archiv für experimentelle Pathologie und Pharmakologie, 1986
- Multiple molecular forms of cyclic nucleotide phosphodiesterase in cardiac and smooth muscle and in plateletsBiochemical Pharmacology, 1986
- Inhibitory action of cyclic GMP on secretion, polyphosphoinositide hydrolysis and calcium mobilization in thrombin-stimulated human plateletsBiochemical and Biophysical Research Communications, 1986
- Mepacrine-induced inhibition of human platelet cyclic-GMP phosphodiesteraseBiochimica et Biophysica Acta (BBA) - General Subjects, 1984
- Purification and characterization of a human platelet cyclic nucleotide phosphodiesteraseBiochemistry, 1984
- EFFECT OF 1-(3-CHLOROANILINO)-4-PHENYLPHTHALAZINE (MY-5445), A SPECIFIC INHIBITOR OF CYCLIC-GMP PHOSPHODIESTERASE, ON HUMAN-PLATELET AGGREGATION1984
- The effects of mepacrine and p-bromophenacyl bromide on arachidonic acid release in human plateletsArchives of Biochemistry and Biophysics, 1982
- Human blood platelet 3′:5′-cyclic nucleotide phosphodiesteraseBiochimica et Biophysica Acta (BBA) - Enzymology, 1976