Incorporation of L‐75Se‐cystine in tissue fragments from the matrix of the hoof and the claw — A tool for studying the pathogenesis of laminitis?
- 1 September 1985
- journal article
- research article
- Published by Wiley in Equine Veterinary Journal
- Vol. 17 (5) , 377-380
- https://doi.org/10.1111/j.2042-3306.1985.tb02527.x
Abstract
An in vitro method has been designed and used to study the incorporation of 75Se-cystine into matrix fragments from hooves and claws of healthy horses and cattle. Tissue fragments from the zone of keratinisation were incubated with L-75Se-cystine in a tissue culture medium for 4 to 6 h, during which time there was continuous incorporation of the labelled selenocystine. The incorporation was greatly decreased by adding L-cystine to the incubation mixture. It is concluded that the incorporation of 75Se-cystine depends on the presence of a specific receptor for cystine in the tissue fragments studied. The possible application of the method to studies of the pathogenesis of laminitis is discussed.This publication has 0 references indexed in Scilit: