Glucose and Cation Transport in Rat Jejunum, Ileum and Colon in Vivo: Control Experiments, and Effect of Cationic Surfactant

Publisher Website
Google Scholar
Abstract
Osmotically balanced solutions of glucose (0.5-300 mM) and NaCl, containing cetrimonium bromide (cetrimide, 0.8-4.1 mM), were instilled into the jejunum, ileum and colon of anesthetized rats. Net transport of glucose, Na and K was studied by their disappearance from, or accumulation into, the intestinal lumen during 15 min incubation. Cetrimide caused the following shifts in normal jejunal and ileal glucose absorption. At low luminal glucose levels, absorption was strongly depressed and may be converted to net secretion. At intermediate levels, inhibition was less pronounced, and at high luminal glucose levels absorption was enhanced. Similar changes were seen in the colon. Cetrimide caused a 3-fold change in the regression lines relating net Na fluxes to the initial Na concentration. The lines became steeper, the correlation was improved and the Na concentration value corresponding to zero net transport was elevated. Net K secretion was increased. These changes are consistent with the view that surfactants cause an increase in passive epithelial permeability. Quantitatively, the effect of cetrimide increased with localization in the order colon > ileum > jejunum. Benzalkonium chloride (0.5-1.7 mM) was tested in the ileum only, and caused quite similar effects.