Mutations in a semiconserved region of the Tetrahymena intron
- 11 March 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 280 (1) , 171-174
- https://doi.org/10.1016/0014-5793(91)80230-z
Abstract
The A-rich in paired region P5a of the Tetrahymena intron is a structural feature that is conserved in the sub-group Ib self-splicing introns. We have constructed a series of substitution and deletion mutations in this region of the intron. Kinetic analysis has shown that some of the mutanis have a reduced maximal extent of splicing, while others have a reduced Vmax. These mutations could be reactivated to a great extent by spermidine and high Mg2+ concentrations. These data are consistent with the hypothesis that the A-rich bulge of P5a has a role in stabilizing the higher-level structure of the ribozymeKeywords
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