SEROLOGY OF PSEUDOMONAS-AERUGINOSA ANALYZED BY MEANS OF QUANTITATIVE IMMUNOELECTROPHORETIC METHODS .5. THERMOSTABILITY, RESISTANCE TO DEGRADATION BY PLASMIN ACTIVITY AND STORAGE CONDITIONS OF A POLYVALENT PSEUDOMONAS-AERUGINOSA REFERENCE STANDARD-ANTIGEN

  • 1 January 1976
    • journal article
    • research article
    • Vol. 84  (5) , 383-389
Abstract
The stability of a polyvalent P. aeruginosa antigen (St-Ag) was analyzed under different experimental conditions by quantitative immunoelectrophoretic methods. St-Ag did not undergo proteolytic degradation by plasmin which is present in the corresponding polyvalent rabbit antiserum (St-Ab) against St-Ag. Storage at -30.degree. C for up to 4 yr caused no qualitative changes in St-Ag and, after 1 yr, only minor quantitative changes could be revealed. Repeated thawing and freezing caused no changes in St-Ag; storage at 4.degree. C or at room temperature caused gradual degradation of most of the antigens of St-Ag beginning after 1 wk. Heating up to 40.degree. C for 2 min did not change St-Ag, but heating for longer periods of time or at higher temperatures gradually destroyed most of the antigens of St-Ag. The most thermostable antigen in St-Ag was number 37, which is related to the group specific O antigens.

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