Expression and characterization of human D4 dopamine receptors in baculovirus‐infected insect cells
- 5 April 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 320 (2) , 130-134
- https://doi.org/10.1016/0014-5793(93)80077-8
Abstract
The human D4 dopamine receptor has been genetically engineered for expression in insect cells using the baculovirus system. A D4 cDNA gene fusion construct [(1991) Nature 350, 610-614] was synthetically modified to remove two introns from the coding region, and expressed in S. frugiperda (Sf9) cells as a fusion with a short sequence from the polyhedrin protein. Binding assays with [3H]spiperone indicated high levels of D4 receptor binding 90 h after infection and a pharmacological profile identical to that reported for D4 receptors expressed in COS-7 cells using the cDNA gene hybrid. We also show that the agonist binding affinity of D4 receptors expressed in Sf9 cells can be shifted by GTP-γ-S, indicating coupling to G-proteins.Keywords
This publication has 16 references indexed in Scilit:
- Multiple dopamine D4 receptor variants in the human populationNature, 1992
- Expression and cell membrane localization of rat M3 muscarinic acetylcholine receptor produced in Sf 9 insect cells using the baculovirus systemFEBS Letters, 1991
- Purification and functional characterization of the human β2‐adrenergic receptor produced in baculovirus‐infected insect cellsFEBS Letters, 1991
- Cloning of the gene for a human dopamine D5 receptor with higher affinity for dopamine than D1Nature, 1991
- Cloning of the gene for a human dopamine D4 receptor with high affinity for the antipsychotic clozapineNature, 1991
- Molecular cloning and expression of the gene for a human D1 dopamine receptorNature, 1990
- High-efficiency expression of mammalian β-adrenergic receptors in baculovirus-infected insect cellsBiochemical and Biophysical Research Communications, 1989
- Trends in the Development of Baculovirus Expression VectorsNature Biotechnology, 1988
- Baculovirus Expression Vectors: the Requirements for High Level Expression of Proteins, Including GlycoproteinsJournal of General Virology, 1987
- Interactions of agonists with D-2 dopamine receptors: Evidence for a single receptor population existing in multiple agonist affinity-states in rat striatal membranesBiochemical Pharmacology, 1984