Lectin binding studies on suspensions of isolated epidermal keratinocytes
- 31 May 1981
- journal article
- research article
- Published by Wiley in British Journal of Dermatology
- Vol. 104 (6) , 641-648
- https://doi.org/10.1111/j.1365-2133.1981.tb00750.x
Abstract
The sugar composition of the surface of isolated human keratinocytes was investigated using lectins. A method is described in which the FITC[fluorescein isothiocyanate]-labeled lectin is displaced with competing sugar prior to measurement. This avoids errors due to aspecific binding and utilizes the minimum biological sample. Little cell surface glycoconjugate apparently is lost during the preparation of the keratinocyte suspension from human epidermal slices using the technique described here. Values for keratinocytes are similar to those reported for fibroblast lines, being much higher than those found using erythrocytes or lymphocytes.This publication has 6 references indexed in Scilit:
- Studies on the plasma membrane of normal and psoriatic keratinocytes.British Journal of Dermatology, 1979
- Studies on the plasma membrane of normal and psoriatic keratinocytes.British Journal of Dermatology, 1979
- Quantitative Fluorimetric Determination of Cell-Surface Glycoconjugates with Fluorescein-Substituted LectinsEuropean Journal of Biochemistry, 1979
- Interactions of plant lectins with glycolipids in liposomesBiochemical and Biophysical Research Communications, 1977
- LECTINSPublished by Elsevier ,1976
- Use of Lectins for the Study of MembranesPublished by Springer Nature ,1975