Effective Diploidization of Microspore‐Derived Haploids of Rape (Brassica napus L.) by In Vitro Colchicine Treatment
- 1 January 1991
- journal article
- Published by Wiley in Plant Breeding
- Vol. 106 (1) , 82-84
- https://doi.org/10.1111/j.1439-0523.1991.tb00483.x
Abstract
A technique for the in vitro colchicine treatment of microspore‐derived haploids of rape (Brassica napus L.) is presented. This allows effective and time‐saving chromosome doubling. The most effective method consists of an application of 50 mg/1 colchicine in a liquid B5 medium to the rooted micro‐spore‐derived plantlets for 4–8 days. By this treatment more than 50 % of the plants developed at least diploid sectors. A change of medium after some days may even increase this diploidization rate.Keywords
This publication has 8 references indexed in Scilit:
- Microspore mutagenesis and selection: Canola plants with field tolerance to the imidazolinonesTheoretical and Applied Genetics, 1989
- Large-scale microspore culture technique for mutation–selection studies in Brassica napusCanadian Journal of Botany, 1988
- An Improved In Vitro Culture Procedure for Embryoids Derived from Isolated Microspores of Rape (Brassica napus L.)Plant Breeding, 1988
- Glucosinolates Determined by HPLC in the Seeds of Microspore-Derived Homozygous Lines of Rapeseed (Brassica napus L.)*Plant Breeding, 1988
- Induction of Haploid Plants From Isolated Pollen of Brassica napusZeitschrift für Pflanzenphysiologie, 1982
- In vitro induction of tetraploid plants from colchicine-treated diploid daylily callusEuphytica, 1979
- Colchicine‐Induced Polyploids from Cell Suspension Cultures of Sugarcane1Crop Science, 1970
- Nutrient requirements of suspension cultures of soybean root cellsExperimental Cell Research, 1968