SONICATION AS A TOOL FOR STUDY OF ADENYLYL CYCLASE ACTIVITY

  • 1 January 1977
    • journal article
    • research article
    • Vol. 3  (3) , 189-198
Abstract
The technique of sonication was applied in studying adenylate cyclase activity of cultured fibroblasts. Exposure of BHK 21 c/13 [baby hamster kidney] to brief periods of low power sonication gave cell preparations with greater basal, fluoride and hormone sensitive adenylate cyclase activities than those of broken cell preparations of homogenized cells. The sonicated cells provided a convenient method to study adenylate cyclase since they were added directly to the adenylate cyclase reaction reaction vessels without further processing. Maximal epinephrine stimulated activity in sonicated cells was nearly equivalent to that activated by NaF, but the apparent affinity of the enzyme system was similar to that of broken cell preparations. Broken cell preparations of sonicated cells possessed greater adenylate cyclase activity than broken cell preparations of unsonicated cells. This procedure may provide a useful tool for the analysis of the hormonal regulation of adenylate cyclase activity of isolated cells.

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