Turbidimetric Changes Accompanying the Interaction of Endotoxin and the α Chains of Human Hemoglobin
- 1 May 1965
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 89 (5) , 1237-1243
- https://doi.org/10.1128/jb.89.5.1237-1243.1965
Abstract
Setlow, Peter (Brandeis University, Waltham, Mass.), Lawrence Levine, and Helen Van Vunakis. Turbidimetric changes accompanying the interaction of endotoxin and the α chains of human hemoglobin. J. Bacteriol. 89:1237–1243. 1965.—With the use of a turbidimetric assay, the effects of pH, ionic strength, anions and cations, and varying concentrations of reactants on the interaction of Escherichia coli endotoxin (prepared by the Boivin procedure) with the α chain of hemoglobin were studied. The reaction was found to be partially reversible by addition of NaCl or by addition of an excess of either reactant. The characteristics of the reaction indicated that complex formation between two multivalent molecules had taken place. The formation of the complex was inhibited by endotoxin (prepared by the Westphal procedure) and by the products which resulted from partial tryptic digestion of α chain.Keywords
This publication has 9 references indexed in Scilit:
- [16] Preparation of buffers for use in enzyme studiesPublished by Elsevier ,2004
- PROPERTIES OF A LYSOZYME-DISSOCIATED ENDOTOXIC FRACTION FROM ESCHERICHIA COLIJournal of Bacteriology, 1964
- Colicine K and endotoxins: Effect of hemoglobin and its subunits on their antibiotic and serological propertiesBiochemical and Biophysical Research Communications, 1964
- Restoration of Endotoxin Activity following Alteration by PlasmaNature, 1964
- Inactivation of Gram-Negative Bacterial Endotoxins by Papain.Experimental Biology and Medicine, 1964
- Reversible inactivation of an endotoxin by intracellular proteinBiochemical Pharmacology, 1963
- STRUCTURE OF HUMAN HEMOGLOBIN .1. SEPARATION OF ALPHA AND BETA CHAINS AND THEIR AMINO ACID COMPOSITION1962
- Interaction of an Endotoxin with Cationic MacromoleculesJournal of General Microbiology, 1961
- Quantitative Micro-Complement Fixation and Its Use in the Study of Antigenic Structure by Specific Antigen-Antibody InhibitionThe Journal of Immunology, 1961