GLycol-EThanol-WAter (3:3:4); A SOlvent for SUdan BLack B STaining

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Abstract
The previously used supersaturated 60% isopropanol method (Lillie 1954, p. 303) protected the more readily soluble lipids from the solvent action of 70% alcohol but precipitation of dye particles within and around sections is sometimes a disturbing complication (cf. Vlachos 1959). The propylene glycol method of Chiffelle and Putt (Lillie, p. 304) has been found to remove much of the birefringent lipid from the sections. Glacial acetic acid dissolves most tissue lipids except those of the solvent-resistant ceroid or lipofuscin type. While solutions in 60–70% acetic acid conserve ordinary fats well (Sills and Marsh 1959) their effect on the more easily soluble lipids should be further explored.

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