Insertion of a retrotransposon within the 3' end of a mouse gene provides a new functional polyadenylation signal

Abstract
A site of genomic insertion of the mouse retrotransposon TLR-IS/MuRRS was analysed. The comparison of the genomic and the cDNA clones indicates the insertion of the LTR-IS element into the 3'' untranslated region of a mous gene. The fact that the isolated cDNA clone ends with a poly A tail 20 nucleotides down stream from the LTR-IS AATAAA box and the result of the S1-nuclease mapping provides evidence that the 3'' end of the mouse gene transcript was generated under the control of the LTR-IS polyadenylation signal.