Laparoscopic Donor Nephrectomy Gene Expression Profiling Reveals Upregulation of Stress and Ischemia Associated Genes Compared to Control Kidneys
- 27 October 2005
- journal article
- research article
- Published by Wolters Kluwer Health in Transplantation
- Vol. 80 (8) , 1067-1071
- https://doi.org/10.1097/01.tp.0000176485.85088.f7
Abstract
We compared gene expression profiles from six donor kidneys prior to surgical manipulation to six kidneys removed after laparoscopic donor nephrectomy (LDN) and several hours of CO2 pneumoperitoneum. Biopsies were obtained from renal cortex and hybridized to Affymetrix HG-U133A GeneChips. For control kidneys, we identified 1380 genes present on all six samples that had a signal intensity >1,000. Functional classification of these revealed genes for cellular signaling (201; 15%), regulation of transcription (156; 11%), cellular transport (144; 10%) and cellular metabolism (111; 8%). A class comparison between the controls and LDN kidneys yielded 865 differentially expressed genes. Functional classification of the 502 genes differentially upregulated in LDN kidneys identified associations with apoptosis, cell adhesion, cell signaling, regulation of cell growth/proliferation, immune/inflammation, ischemia/stress response and proteolysis/peptidolysis. These data demonstrate an altered renal transcriptome induced by several hours of CO2 pneumoperitoneum and laparoscopic surgery characterized by upregulation of ischemia and injury associated genes.Keywords
This publication has 20 references indexed in Scilit:
- Alterations in Gene Expression in Cadaveric vs. Live Donor Kidneys Suggest Impaired Tubular Counterbalance of Oxidative Stress at ImplantationAmerican Journal of Transplantation, 2004
- Laparoscopic Donor Nephrectomy: The University of Maryland 6-Year ExperienceJournal of Urology, 2004
- Identification of Renal Progenitor-Like Tubular Cells that Participate in the Regeneration Processes of the KidneyJournal of the American Society of Nephrology, 2003
- Role of hypoxia inducible factors 1α and 2α in basal adhesion formation and in carbon dioxide pneumoperitoneum-enhanced adhesion formation after laparoscopic surgery in transgenic miceFertility and Sterility, 2003
- Cutting Edge: The B Cell Chemokine CXC Chemokine Ligand 13/B Lymphocyte Chemoattractant Is Expressed in the High Endothelial Venules of Lymph Nodes and Peyer’s Patches and Affects B Cell Trafficking Across High Endothelial VenulesThe Journal of Immunology, 2003
- CO2 Pneumoperitoneum Modifies the Inflammatory Response to SepsisAnnals of Surgery, 2003
- Increased expression of P-cadherin mRNA in the mouse peritoneum after carbon dioxide insufflationSurgical Endoscopy, 2001
- Damage-induced neuronal endopeptidase (DINE) is a unique metallopeptidase expressed in response to neuronal damage and activates superoxide scavengersProceedings of the National Academy of Sciences, 2000
- Cluster analysis and display of genome-wide expression patternsProceedings of the National Academy of Sciences, 1998
- Renal Tubular Antiproteinase (Alpha-1-Antitrypsin and Alpha-1-Antichymotrypsin) Response in Tubulo-Interstitial DamageNephron, 1993