Detection of transcriptionally active hepatitis B virus DNA in peripheral mononuclear blood cells after infection during immunosuppressive chemotherapy using the polymerase chain reaction
- 1 January 1992
- book chapter
- Published by Springer Nature
- Vol. 4, 50-53
- https://doi.org/10.1007/978-3-7091-5633-9_11
Abstract
Using PCR we have studied mononuclear peripheral blood leucocytes (PMBLs) from HBV-infected immunosuppressed patients in order to detect the presence of HBV genomes. Our results indicate that non-transient PMBL infection is common in immunotolerant carriers. In addition, the presence of pregenomic mRNA sequences suggests that virus replication may take place in PMBLs, possibly implicating the latter as a source of virus after replication has ceased in the liver.Keywords
This publication has 9 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- PERSISTENT HEPATITIS B VIRUS INFECTION OF MONONUCLEAR BLOOD CELLS WITHOUT CONCOMITANT LIVER INFECTIONTransplantation, 1990
- Hepatitis B virus infection of peripheral blood mononuclear cells is common in acute and chronic hepatitisJournal of Medical Virology, 1990
- Hepatitis B virus DNA in peripheral blood leukocytes: A brief reviewJournal of Medical Virology, 1990
- TRANSMISSION OF HEPATITIS B FROM HEPATITIS-B-SERONEGATIVE SUBJECTSThe Lancet, 1988
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- The hepatitis B virusNature, 1985
- Subtype ayw variant of hepatitis B virusFEBS Letters, 1985
- Hepatitis B viral DNA-RNA hybrid molecules in particles from infected liver are converted to viral DNA molecules during an endogenous dna polymerase reactionVirology, 1984