Microsomal palmitoyl-coenzyme A synthetase from rat liver. Partial and exchange reactions
- 1 October 1972
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 129 (5) , 1101-1107
- https://doi.org/10.1042/bj1291101
Abstract
The partial and exchange reactions of long-chain fatty acid activation were determined by using purified microsomal long-chain fatty acyl-CoA synthetase (EC 6.2.1.3). No significant ATP formation from palmitoyl-AMP and PPi, nor palmitoyl-AMP-dependent CoA disappearance could be demonstrated. Similarly, no palmitate-dependent [32P2]PPi–ATP exchange was catalysed by the pure enzyme. The above partial and exchange reactions were, however, catalysed by the parent microsomal fraction at a rate similar to that of the overall reaction. The implications of these results are discussed.Keywords
This publication has 15 references indexed in Scilit:
- Rate law and mechanism of the adenosine triphosphate-pyrophosphate isotope exchange reaction of amino acyl transfer ribonucleic acid synthetasesBiochemistry, 1970
- Induced Activation of Amino Acid Activating Enzymes by Amino Acids and †RNAProgress in Nucleic Acid Research and Molecular Biology, 1970
- Substrate synergism and phosphoenzyme formation in catalysis by succinyl coenzyme A synthetaseBiochemistry, 1968
- Studies on medium-chain fatty acyl-coenzyme A synthetase. Purification and propertiesBiochemical Journal, 1968
- Studies of medium-chain fatty acyl-coenzyme A synthetase. Enzyme fraction II: mechanism of reaction and specific propertiesBiochemical Journal, 1968
- Studies on medium-chain fatty acyl-coenzyme A synthetase. Enzyme fraction I: mechanism of reaction and allosteric propertiesBiochemical Journal, 1968
- Studies of the formation and utilization of bound CoA in glyceride biosynthesisBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1967
- A Coenzyme A analogue, Desulpho-CoA; Preparation and effects on various enzymesBiochemical and Biophysical Research Communications, 1966
- Acyl carrier protein. V. Identification of 4'-phosphopantetheine bound to a mammalian fatty acid synthetase preparation.Proceedings of the National Academy of Sciences, 1965
- Studies on palmitoyl-coenzyme A synthetaseBiochemical Journal, 1964