Combination of IL-8 Plus TNFα Induces Additive Neutrophil Migration

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Abstract
We have previously reported that TNFα's ability to induce neutrophil transendothelial and transepithelial migration was largely dependent on the generation of IL-8 by the endothelial or pulmonary epithelial cells. To further explore the interrelationship of IL-8 with TNFα, we examined the migration of human neutrophils through noncellular barriers in response to these cytokines alone or in combination. We directly compared neutrophil migration through 3 m-pore size polycarbonate Transwell filters in response to 10 nM TNFα or either 10 nM TNFα or buffer plus 10−8 to 10−11 M IL-8. We found that the combination of TNFα and IL-8 induced neutrophil migration that was generally additive, and sometimes synergistic, to that of either agent alone. In conclusion, these data support the role of cytokine networking in inducing neutrophil-rich lung inflammatory responses.

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