Urinary 17-Hydroxycorticosteroid Determination with p-Hydrazinobenzenesulfonic Acid–Phosphoric Acid
- 1 November 1973
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 60 (5) , 684-690
- https://doi.org/10.1093/ajcp/60.5.684
Abstract
A new reagent system with p-hydrazinobenzenesulfonic acid in phosphoric acid for the determination of urinary 17-hydroxycorticosteroids is described. Use of this reagent results in increased sensitivity of detection of compounds possessing a dihydroxyacetone side chain while it retains the same specificity as obtained with the Porter-Silber reagent. The reaction conditions employed with the new reagent allow the assay to be performed within 4 to 8 hours after hydrolysis of the samples of urine.Keywords
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