Selection of reference genes for qRT-PCR examination of wild populations of Atlantic cod Gadus morhua
Open Access
- 1 January 2008
- journal article
- Published by Springer Nature in BMC Research Notes
- Vol. 1 (1) , 47
- https://doi.org/10.1186/1756-0500-1-47
Abstract
Extensive sequencing efforts have been taking place for the Atlantic cod (Gadus morhua) in recent years, the number of ESTs in the Genbank has reached more than 140.000. Despite its importance in North Atlantic fisheries and potential use in aquaculture, relatively few gene expression examination exists for this species, and systematic evaluations of reference gene stability in quantitative real-time RT-PCR (qRT-PCR) studies are lacking.Keywords
This publication has 13 references indexed in Scilit:
- Selection of housekeeping genes for gene expression studies in larvae from flatfish using real-time PCRBMC Molecular Biology, 2008
- Development and Validation of Endogenous Reference Genes for Expression Profiling of Medaka (Oryzias latipes) Exposed to Endocrine Disrupting Chemicals by Quantitative Real-Time RT-PCRToxicological Sciences, 2006
- Selection of reference genes for quantitative RT-PCR studies in striped dolphin (Stenella coeruleoalba) skin biopsiesBMC Molecular Biology, 2006
- Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmonBMC Molecular Biology, 2005
- Quantitative real-time RT-PCR – a perspectiveJournal of Molecular Endocrinology, 2005
- Real-time RT-PCR normalisation; strategies and considerationsGenes & Immunity, 2005
- Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infectionsVirology Journal, 2005
- Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlationsBiotechnology Letters, 2004
- Guideline to reference gene selection for quantitative real-time PCRPublished by Elsevier ,2003
- Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genesGenome Biology, 2002