Studies on a paramyxovirus isolated from Japanese sparrow-hawks (Accipiter virgatus gularis). IV. Hemagglutinating activity of two clones of the virus.

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Abstract
The Takavirus, a variant of Newcastle disease virus (NDV), was found to agglutinate chicken erythrocytes unstably at room temperature. Two clones were obtained from the plaques formed in the chick embryo fibroblasts (CEF); 1 showed a constant hemagglutinating (HA) reaction only at 4.degree. C (L+R-); the other showed no significant reversion in the HA reaction at 4.degree. C or at room temperature (L+R+). There was no difference in the structural polypeptides and serological identity among 2 clones of Takavirus and 2 strains of NDV. The hemolytic activity of the Takaviruses (L+R-, L+R+ and parent) was similar to that of NDV-Sato strain, but much lower than that of NDV-B1 strain. The elution of L+R- from the chicken erythrocytes occurred more rapidly at room temperature. The amount of the eluted virus was greater than that obtained from L+R+ or NDV. The reaction did not occur when fresh viruses were added to the erythrocytes which had eluted the viruses.

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