Affinity chromatography purification of type A botulinum neurotoxin from crystalline toxic complex
- 1 May 1978
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 35 (5) , 878-880
- https://doi.org/10.1128/aem.35.5.878-880.1978
Abstract
Type A botulinum neurotoxin was purified from toxic crystals by adsorption to p-aminophenyl-beta-D-thiogalactopyranoside coupled to CH-Sepharose 4B. At pH 6.3, the toxic complex was held by the binding between the ligand and the hemagglutinin of the complex; the toxin is eluted selectively by dissociating the complex with buffer-saline of pH 7.9. The single-step affinity chromatography recovered 50 to 60% of applied toxicity as preparations of greater than 99% purity.This publication has 13 references indexed in Scilit:
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