Preparation of Australia Antigen Free Human Albumin with Polyethylene Glycol

1 January 1973

journal article
research article
Published by Taylor & Francis in Preparative Biochemistry

Vol. 3 (1) , 31-45
https://doi.org/10.1080/00327487308061487

Abstract

Albumin was prepared from human plasma diluted In 0.05M phosphate buffer pH 8.6, by the precipitation of approximately 95% of the associated plasma proteins with 357. polyethylene glycol. More than 70% of the albumin In the original plasma was recoverable as a viscous liquid on lowering the pH to 5.5. The albumin prepared by this technique is associated with 5 to 10% or and B globulin. Plasma, positive for Australia antigen (Au(SH)ag) yields an albumin preparation negative for the antigen.

Keywords

This publication has 2 references indexed in Scilit:

Recovery of Australia Antigen from Human Plasma Products Separated by a Modified Cohn Fractionation
Vox Sanguinis, 1972
The fractionation of protein mixtures by linear polymers of high molecular weight
Biochimica et Biophysica Acta (BBA) - General Subjects, 1964