IDENTIFICATION OF CELLS EXPRESSING T AND P28,33 (IA-LIKE) ANTIGENS IN SECTIONS OF HUMAN LYMPHOID-TISSUE

  • 1 January 1980
    • journal article
    • research article
    • Vol. 39  (1) , 66-75
Abstract
A method was developed for identifying human lymphocyte populations in tissue sections using hetero-antisera to T [thymus-derived] cells and to the B [bone marrow-derived] cell associated Ia[immune response associated antigen]-like, p28, 33 polypeptide complex. The differential topographical localization of T and B cells was revealed by simultaneous staining with antibodies tagged with different fluorochromes. This approach also identified a small population of T cells bearing Ia-like antigens. Preliminary studies on lymphomatous tissue suggests this approach will be of value in the study of malignancy and cellular infiltration in disease.