PHOTOREACTIONS OF ISORHODOPSIN AT LOW TEMPERATURES

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Abstract
Abstract— The photoreactions of isorhodopsin, a synthetic visual pigment containing 9‐cis retinal, were studied between –45° and –75°C using flash photolysis. Light‐induced absorbancy changes were observed which were indistinguishable from those caused by the photo‐production of prelumirhodopsin from the natural visual pigment rhodopsin. It is concluded that isorhodopsin is converted to prelumirhodopsin although less efficiently than is the natural pigment. Thermally unstable intennediates of bleaching are also converted to pre‐lumirhodop‐sin by light, possibly by way of rhodopsin or isorhodopsin.

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