THE FORMATION OF PLAQUES BY TWO STRAINS OF VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS1

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Abstract
Monolayers of chick-embryo fibroblasts and L cells inoculated with the parent VE-1 strain of Venezuelan equine encephalomyelitis virus yielded macroscopic plaques after 48 hours of incubation while cell sheets inoculated with the variant CILV-8 strain required at least 72 hours. Diameters of plaques produced by the VE-1 and the CILV-8 strains were 3.0 to 4.5 and 0.5 to 1 millimeters in diameter, respectively. The suspended cell plaque and the mouse intracerebral injection methods of assay showed comparable titers with the VE-1 strain. They were 10 times more sensitive as methods of assay than either the monolayer plaque or mouse intraperitoneal injection methods. The monolayer plaque and the mouse intracerebral injection methods of assay of the CILV-8 strain were equal in sensitivity to one another.

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