Escherichia coli Physiology in Luria-Bertani Broth
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- 1 December 2007
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 189 (23) , 8746-8749
- https://doi.org/10.1128/jb.01368-07
Abstract
Luria-Bertani broth supports Escherichia coli growth to an optical density at 600 nm (OD 600 ) of 7. Surprisingly, however, steady-state growth ceases at an OD 600 of 0.3, when the growth rate slows down and cell mass decreases. Growth stops for lack of a utilizable carbon source. The carbon sources for E. coli in Luria-Bertani broth are catabolizable amino acids, not sugars.Keywords
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