A Rapid Method for the Quantitative Study of RNA from Canine Distemper Virus Infected Cells

1 August 1976

journal article
research article
Published by Microbiology Society in Journal of General Virology

Vol. 32 (2) , 321-325
https://doi.org/10.1099/0022-1317-32-2-321

Abstract

Centrifugation through CsCl was used to isolate 32P-labeled RNA in a 1-step purification procedure. The method is suitable for quantitative and preparative studies and appears to have considerable advantages over conventional methods of RNA extraction. This procedure was used to investigate the RNA synthesized in Vero [African Green monkey kidney] cells infected with canine distemper virus (CDV). The combination of CsCl centrifugation and affinity chromatography on poly-U Sepharose provides a rapid method for isolating mRNA from virus infected cells.

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