Detection of Normal and Malignant Megakaryocytes by Anti β-Thromboglobulin Serum

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Abstract
An antiserum to .beta.-thromboglobulin was used in immunofluorescence to detect normal and malignant [human] megakaryocytes. In normal peripheral blood and bone marrow smears, only platelets and megakaryocytes were specifically stained by this antiserum. Among 25 cases of acute lymphoid and nonlymphoid leukemia, only 2 exhibited a clear positivity in a percentage of blasts, thus proving their megakaryocytic origin. This method is proposed as a simple and useful tool to detect normal and malignant megakaryoctes on smears.