Autocatalytic cyclization of an excised intervening sequence RNA is a cleavage–ligation reaction
- 1 February 1983
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 301 (5901) , 578-583
- https://doi.org/10.1038/301578a0
Abstract
The intervening sequence (IVS) of the Tetrahymena rRNA precursor is excised as a linear RNA molecule which subsequently cyclizes itself in a protein-independent reaction. Cyclization involves cleavage of the linear IVS RNA 15 nucleotides from its 5'' end and formation of a phosphodiester bond between the new 5'' phosphate and the original 3''-hydroxyl terminus of the IVS. This recombination mechanism is analogous to that by which splicing of the precursor RNA is achieved. The circular molecules appear to have no direct function in RNA splicing, and the cyclization evidently serves to prevent unwanted RNA from driving the splicing reactions backwards.Keywords
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