Overexpression, purification and crystallization ofBamHI endonuclease
- 25 April 1991
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 19 (8) , 1825-1829
- https://doi.org/10.1093/nar/19.8.1825
Abstract
The type II restriction endonuclease Bam HI has been expressed In E. coli , producing 100-fold more enzyme than the wild type Bacillus amyloliquefaciens H strain. This high yield has facilitated purification to homogeneity of large amounts of the enzyme, along with its crystallization in a form which diffracts to at least 1.9 A in X-ray analysis.Keywords
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