Detection of Four Isometric Plant Viruses in Sodium Dodecyl Sulfate Immunodiffusion Tests

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Abstract
Antisera were obtained by immunizing rabbits with purified, freeze-dried sodium dodecyl sulfate(SDS)-treated preparations of cowpea mosaic, cucumber mosaic, southern bean mosaic and squash mosaic (SqMV) viruses. Each antiserum reacted homologously in SDS-immunodiffusion tests against SDS-treated extracts of infected leaves that were heated in a boiling water bath for 4 min prior to use. None of the antisera to the isometric viruses cross-reacted in reciprocal tests. An antiserum to untreated SqMV reacted specifically in SDS-immunodiffusion tests with either aqueous extracts or SDS-treated, nonheated sap, but did not react with heated, SDS-treated extracts. SDS-immunodiffusion tests are feasible for diagnosis of certain spherical viruses under conditions in which filamentous plant viruses also can readily be detected.

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