Specific binding of radioiodinated granulocyte-macrophage colony-stimulating factor to hemopoietic cells.
Open Access
- 1 April 1985
- journal article
- research article
- Published by Springer Nature in The EMBO Journal
- Vol. 4 (4) , 933-939
- https://doi.org/10.1002/j.1460-2075.1985.tb03721.x
Abstract
The hemopoietic growth factor granulocyte‐macrophage colony‐stimulating factor, GM‐CSF, specifically controls the production of granulocytes and macrophages. This report describes the binding of biologically‐active 125I‐labeled murine GM‐CSF to a range of hemopoietic cells. Specific binding was restricted to murine cells and neither rat nor human bone marrow cells appeared to have surface receptors for 125I‐labeled GM‐CSF. 125I‐Labeled GM‐CSF only appeared to bind specifically to cells in the myelomonocytic lineage. The binding of 125I‐labeled GM‐CSF to both bone marrow cells and WEHI‐3B(D+) was rapid (50% maximum binding was attained within 5 min at both 20 degrees C and 37 degrees C). Unlabeled GM‐CSF was the only polypeptide hormone which completely inhibited the binding of 125I‐labeled GM‐CSF to bone marrow cells, however, multi‐CSF (also called IL‐3) and G‐CSF partially reduced the binding of 125I‐labeled GM‐CSF to bone marrow cells. Interestingly, the binding of 125I‐labeled GM‐CSF to a myelomonocytic cell line, WEHI‐3B(D+), was inhibited by unlabeled GM‐CSF but not by multi‐CSF or G‐CSF. Scatchard analysis of the binding of 125I‐labeled GM‐CSF to WEHI‐3B(D+) cells, bone marrow cells and peritoneal neutrophils indicated that there were two classes of binding sites: one of high affinity (Kd1 = 20 pM) and one of low affinity (Kd2 = 0.8‐1.2 nM). Multi‐CSF only inhibited the binding of 125I‐labeled GM‐CSF to the high affinity receptor on bone marrow cells: this inhibition appeared to be a result of down regulation or modification of the GM‐CSF receptor.(ABSTRACT TRUNCATED AT 250 WORDS)This publication has 46 references indexed in Scilit:
- Molecular cloning of cDNA encoding a murine haematopoietic growth regulator, granulocyte—macrophage colony stimulating factorNature, 1984
- Molecular cloning of cDNA for murine interleukin-3Nature, 1984
- Erythropoietin causes down regulation of colony-stimulating factor (CSF-1) receptors on peritoneal exudate macrophages of the mouse.The Journal of cell biology, 1983
- Interactions between the receptors for platelet-derived growth factor and epidermal growth factor.The Journal of cell biology, 1983
- T cell growth factor receptors. Quantitation, specificity, and biological relevanceThe Journal of Experimental Medicine, 1981
- Isolation and surface labeling of murine polymorphonuclear neutrophilsJournal of Cellular Physiology, 1979
- Functional macrophage cell lines transformed by abelson leukemia virusCell, 1978
- Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphenylglycolurilBiochemical and Biophysical Research Communications, 1978
- Selective in Vitro Growth of T Lymphocytes from Normal Human Bone MarrowsScience, 1976
- Tissue sources of bone marrow colony stimulating factorJournal of Cellular Physiology, 1971