DIFFERENCES IN THE AMINO ACID COMPOSITION OF TWO PURIFIED ANTIBODIES FROM THE SAME RABBIT

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Abstract
Small but significant differences were found in the amino acid composition of 2 antibodies purified from pooled sera or from the serum of the same rabbit. The antibody directed against the negatively charged p-azo-benzenearsonic acid group had a higher arginine and isoleucine content, while the antibody directed against the positively charged p-azophenyltrimethylammonium ion had a higher aspartic acid and leucine content. The similarity in the recoveries of the remaining amino acids was striking, and the molecular weights of the 2 antibodies were identical within the limits of the method. The possible relationship of these differences in amino acid composition to antibody specificity and to the microheterogeneity of gamma globulin is discussed.