Complete Nucleotide Sequence of Tn 10

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Abstract
The complete nucleotide sequence of Tn10 has been determined. The dinucleotide signature and percent G+C of the sequence had no discontinuities, indicating that Tn10 constitutes a homogeneous unit. The new sequence contained three new open reading frames corresponding to a glutamate permease, repressors of heavy metal resistance operons, and a hypothetical protein inBacillus subtilis. The glutamate permease was fully functional when expressed, but Tn10 did not protectEscherichia coli from the toxic effects of various metals.