MIGRATION OF LEUKOCYTES TO HUMAN NASAL-MUCOSA

  • 1 January 1978
    • journal article
    • research article
    • Vol. 92  (4) , 556-559
Abstract
To perform their antimicrobial function PMN [polymorphonuclear neutrophils] must be able to migrate from capillaries to tissues and mucusal surfaces. Cellophane squares placed against the nasal septum can stimulate a local cellular response resulting in the migration of granulocytes to the surface of the squares. Upon removal of the squares, adherent cells can be stained, identified and quantitated. Studies in which the cellophane squares are left against the septum for 5 min reveal a dense monolayer of granulocytes (greater than 6/70 .mu.m2) on only 7% .+-. 2 of the surface of the square. After 2 h against the nasal septum, a dense monolayer of granulocytes was found on 87% .+-. 10 of the surface of the cellophane square. This technique provides a simple and noninvasive method for in vivo measurement of leukocyte migration.

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