PARTIAL CHARACTERIZATION OF HUMAN COLLAGEN AND PROCOLLAGEN SECRETED BY HUMAN CORNEAL STROMAL FIBROBLASTS IN CELL-CULTURE
- 1 January 1978
- journal article
- research article
- Vol. 17 (3) , 264-271
Abstract
Human corneal stromal fibroblasts were established into cell culture. Fractions highly enriched for collagen and procollagen were purified from the medium and cell layer. Analysis using polyacrylamide gel electrophoresis and DEAE cellulose chromatography indicated the presence of a heterogeneous population of procollagen molecules of MW 200,000-120,000 daltons along with .alpha.1 and .alpha.2 collagen chains. Cyanogen bromide peptides of a corneal peptide fraction migrated concomitantly with type I standard cyanogen bromide peptides.This publication has 3 references indexed in Scilit:
- Procollagen and collagen produced by a teratocarcinoma-derived cell line, TSD4: Evidence for a new molecular form of collagenCell, 1977
- Biochemical characteristics and biological significance of the genetically-distinct collagensMolecular and Cellular Biochemistry, 1976
- Comparison of collagen preparations from beef cornea and scleraBiochimica et Biophysica Acta, 1957