Temperature-dependent template switching duringin vitrocDNA synthesis by the AMV-reverse transcriptase
- 1 January 1992
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 20 (20) , 5443-5450
- https://doi.org/10.1093/nar/20.20.5443
Abstract
Reverse transcriptase template switching has been invoked to explain several aspects of retroviral replication and recombination, and has been reported in vitro for the Moloney murine leukemia virus (M-MuLV) reverse transcriptase. During in vitro cDNA synthesis, the avian myeloblastosis virus (AMV) reverse transcriptase can switch from one template to another in a homology-dependent and temperature-dependent manner. Chimeric cDNA molecules are generated within 30 min at high incubation temperatures, with an increasing efficiency from 42 degrees C to 50 degrees C. Such products are detectable only after much longer incubation times when primer extension reactions are carried out at lower temperatures (90 min at 37 degrees C).Keywords
This publication has 11 references indexed in Scilit:
- Sex and recombination in retrovirusesTrends in Genetics, 1991
- Nucleotide sequence and control of transcription of the bacteriophage T4motAregulatory geneMolecular Microbiology, 1990
- INTRINSIC-PROPERTIES OF REVERSE-TRANSCRIPTASE IN REVERSE TRANSCRIPTION - ASSOCIATED RNASE H IS ESSENTIALLY REGARDED AS AN ENDONUCLEASE1989
- Transcriptional activation of bacteriophage T4 middle promoters by the motA proteinJournal of Molecular Biology, 1988
- High-frequency deletion between homologous sequences during retrotransposition of Ty elements in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences, 1987
- Recombination between bacteriophage T4 and plasmid pBR322 molecules containing cloned T4 DNAJournal of Molecular Biology, 1983
- Transduction of a cellular oncogene: the genesis of Rous sarcoma virus.Proceedings of the National Academy of Sciences, 1983
- Analysis of gene control signals by DNA fusion and cloning in Escherichia coliJournal of Molecular Biology, 1980
- Isolation and characterization of conditional lethal mutations in the mot gene of bacteriophage T4Journal of Molecular Biology, 1978
- Mutant of bacteriophage T4D affecting expression of many early genesNature, 1974