Physical properties of the dimyristoylphosphatidylcholine vesicle and of complexes formed by its interaction with apolipoprotein C-III

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Abstract
The structure of a single bilayer vesicle of dimyristoylphosphatidylcholine was characterized by sedimentation, densimetry and light-scattering measurements. The MW, partial specific volume, Stokes radius and degree of hydration were found to be 2.68 .times. 106, 0.972 cm3/g, 125 .ANG. and 0.86 g/g, respectively. From these quantities, a spherically symmetrical model was derived that features a phospholipid bilayer 35.5 .ANG. thick and a hydration shell 9.3 .ANG. thick. This particle bound [human very low density lipoprotein] apolipoprotein C-III (apoC-III) up to 0.08 g/g without loss of its original vesicular structure. At protein-lipid ratios in excess of 0.08 g/g, sedimentation, gel chromatography and light-scattering measurements indicated a dramatic decrease in Stokes radius and MW. The sedimentation data showed these parameters to become constant at protein-lipid ratios in excess of 0.25 g/g. In this region, the Stokes radius and MW were .apprx. 80 .ANG. and 442,000, respectively. Within the constraints of these values and other data, several models for this complex are discussed.

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