BENZO(A)PYRENE HYDROXYLASE-ACTIVITY IN ENRICHED POPULATIONS OF CLARA CELLS AND ALVEOLAR TYPE-II CELLS FROM CONTROL AND BETA-NAPHTHOFLAVONE-PRETREATED RATS
- 1 January 1982
- journal article
- research article
- Vol. 42 (11) , 4658-4663
Abstract
A method was developed for isolation and purification of specific types of lung cells from rats. Alveolar type II cells were purified from 18% in the initial cell digest to 30% after centrifugal elutriation and finally to over 80% following density gradient centrifugation. Clara cells were enriched from 0.8% in the cell digest to 40-60% by use of centrifugal elutriation. In control animals, aryl hydrocarbon hydroxylase activity was found to increase in parallel with Clara cell purity but was almost undetectable in the type II cells. Following pretreatment of rats with .beta.-naphthoflavone, aryl hydrocarbon hydroxylase activity increased both in Clara cells and particularly, in alveolar type II cells. This methodology provides a means for investigation of the effects of toxic and carcinogenic compounds on different populations of lung cells.This publication has 7 references indexed in Scilit:
- INVITRO METABOLIC-ACTIVATION OF THE PULMONARY TOXIN, 4-IPOMEANOL, IN NON-CILIATED BRONCHIOLAR EPITHELIAL (CLARA) AND ALVEOLAR TYPE-II CELLS ISOLATED FROM RABBIT LUNG1982
- Xenobiotic metabolism by alveolar type II cells isolated from rabbit lungBiochemical Pharmacology, 1981
- A SCANNING ELECTRON-MICROSCOPIC STUDY OF THE TYPE-II AND CLARA CELL ADENOMA OF THE MOUSE LUNG1980
- PULMONARY CLARA CELL AS A TARGET FOR TOXIC-CHEMICALS REQUIRING METABOLIC-ACTIVATION - STUDIES WITH CARBON-TETRACHLORIDE1980
- FOREIGN COMPOUND METABOLISM BY ISOLATED CELLS FROM RABBIT LUNG1979
- HISTOLOGIC AND ULTRASTRUCTURAL FEATURES OF THE CLARA CELL ADENOMA OF THE MOUSE LUNG1979
- STUDIES USING SEDIMENTATION-VELOCITY ON HETEROGENEITY OF SIZE AND FUNCTION OF HEPATOCYTES FROM MATURE MALE RATS1978