Microfluorometric studies on chromosomes
- 1 November 1983
- journal article
- research article
- Published by Springer Nature in Chromosoma
- Vol. 88 (5) , 374-376
- https://doi.org/10.1007/bf00285859
Abstract
The fluorescent stains, dansyl chloride and fluorescamine, were used to indicate the amount of protein in G-banded and unbanded chromosomes 1 of the Chinese hamster relative to the average amount of protein in human erythrocyte. Fluorescence was found to be proportional to protein mass up to the equivalent of three erythrocytes. In G-banding, trypsin digestion resulted in an average protein loss of 35.4% compared with unbanded chromosomes.Keywords
This publication has 9 references indexed in Scilit:
- MECHANISMS OF CHROMOSOME BANDING AND IMPLICATIONS FOR CHROMOSOME STRUCTUREAnnual Review of Genetics, 1978
- Histones removed by fixationExperimental Cell Research, 1977
- Mechanisms of chromosome banding: II. Evidence that histones are not involvedExperimental Cell Research, 1974
- Histones in cytological preparationsExperimental Cell Research, 1974
- Mechanisms involved in the banding of chromosomes with quinacrine and Giemsa: I. The effects of fixation in methanol-acetic acidExperimental Cell Research, 1973
- Fluorescamine: A Reagent for Assay of Amino Acids, Peptides, Proteins, and Primary Amines in the Picomole RangeScience, 1972
- [8] End-group analysis using dansyl chloridePublished by Elsevier ,1972
- The effect of two acetic acid containing fixatives on the histone content of calf thymus deoxyribonucleoprotein and calf thymus tissueExperimental Cell Research, 1968
- Observations on the Chromosomes of Chinese Hamster Cells in Tissue Culture2JNCI Journal of the National Cancer Institute, 1958