Preparation and Regeneration of Protoplasts from Axenic Mycelia Derived from the Wheat Stem Rust Fungus
- 1 January 1990
- journal article
- research article
- Published by Scientific Societies in Phytopathology®
- Vol. 80 (1) , 81-84
- https://doi.org/10.1094/phyto-80-81
Abstract
Protoplasts were prepared for axenic suspension cultures of the wheat stem rust fungus Puccinia graminis f. sp. tritici, race 126 Anz-5,6,7,11. Protoplats were released in 3 hr from 3- to 7-day-old mycelia with a mixture of Novozym 234 and cellulase (5 mg each/ml) osmotically stablized by 0.8 M MgSO4. Protoplast regeneration was detected microscopically within 10 hr after incubation in a nutrient medium containing 0.5 M sorbitol, and colonies usually were visible after a further 3 days of growth. The regeneration frequency ranged from 19 to 39%.This publication has 8 references indexed in Scilit:
- Characterization of an infection structure-specific gene from the rust fungus Uromyces appendiculatusGene, 1989
- A Mechanosensitive Ion Channel in the Yeast Plasma MembraneScience, 1988
- Gene transfer system for the phytopathogenic fungus Ustilago maydis.Proceedings of the National Academy of Sciences, 1988
- Signaling for Growth Orientation and Cell Differentiation by Surface Topography in UromycesScience, 1987
- Transformation of the basidiomycete,Schizophyllum communeMolecular Genetics and Genomics, 1986
- Hexitols as major intermediates of glucose assimilation by mycelium of Puccinia graminisArchiv für Mikrobiologie, 1984
- A possible role for microtubules and microfilaments in the induction of nuclear division in bean rust uredospore germlingsExperimental Mycology, 1982
- Identification of Glucitol (Sorbitol) and Ribitol in a Rust Fungus, Puccinia graminis f. sp. triticiJournal of General Microbiology, 1976