IMMUNOCHEMISTRY OF I/I-ACTIVE OLIGOGLYCOSYLCERAMIDES AND POLYGLYCOSYLCERAMIDES FROM RABBIT ERYTHROCYTE-MEMBRANES - DETERMINATION OF BRANCHING PATTERNS OF A CERAMIDE PENTADECASACCHARIDE BY H-1 NUCLEAR MAGNETIC-RESONANCE

  • 1 January 1984
    • journal article
    • research article
    • Vol. 259  (12) , 7648-7651
Abstract
1H NMR spectra of the ceramide hexasaccharide obtained after the removal of the terminal .alpha.-Gal and subterminal .beta.-Gal residues from the ceramide decasaccharide showed that terminal and internal GlcNAc residues are differentiated by their chemical shifts. The primary structure of the title compound was determined. Alternative branching of this oligosaccharide chain was excluded since the removal of all terminal .alpha.-Gal and penultimate .beta.-Gal residues yielded a ceramide nonasaccharide containing 1 terminal and 2 internal 1 .fwdarw. 3-linked GlcNAc residues, as well as 2 terminal 1 .fwdarw. 6-linked GlcNAc units. The intermediate degradation products of the ceramide deca- and pentadecasaccharides, namely the ceramide octa- and dodecasaccharide, obtained by the removal of .alpha.-Gal residues only, as well as the linear ceramide heptasaccharide and ceramide hexasaccharide were also investigated. The usefulness of the glycosylation-induced chemical shifts is discussed.

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