Unusual Alkaline Elution Pattern Induced in Mammalian Cell DNA by Fast Neutrons p(34) + Be

1 January 1988

journal article
research article
Published by Taylor & Francis in International Journal of Radiation Biology

Vol. 54 (1) , 39-53
https://doi.org/10.1080/09553008814551491

Abstract

Syrian hamster fibroblasts (cell line BHK 21/13) were exposed to p(34)+Be fast neutron irradiation and their DNA analysed by the alkaline elution technique. The elution profiles showed an unusual tailing off, characteristic of neutron-irradiated samples, suggesting the presence of a modification in DNA induced by the neutrons. This was not seen with 60Co gamma-irradiation. In neutron-irradiated samples the alteration of DNA appeared to persist even after 2 h of post-treatment incubation (37.degree.C) indicating the absence of repair. The modification of DNA induced by neutrons provides a possible explanation for the reduction of the shoulders in survival curves obtained with neutrons, and the high RBF of neutrons.

This publication has 14 references indexed in Scilit:

Chemical Changes Induced in DNA by Ionizing Radiation
Progress in Nucleic Acid Research and Molecular Biology, 1985
EVALUATION OF DNA CROSSLINKS AND MONOADDUCTS IN MOUSE EMBRYO FIBROBLASTS AFTER TREATMENT WITH MONO-AND BIFUNCTIONAL FUROCOUMARINS AND 365 nm (UVA) IRRADIATION. POSSIBLE RELATIONSHIP TO CARCINOGENICITY
Photochemistry and Photobiology, 1983
Rapid Rejoining of DNA Strand Breaks in Resting Human Lymphocytes after Irradiation by Low Doses of 60 Co g Rays or 14.6-MeV Neutrons
Radiation Research, 1983
European protocol for neutron dosimetry for external beam therapy
The British Journal of Radiology, 1981
DNA crosslinking induced by X-rays and chemical agents
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1977
High-LET radiations induce a large proportion of non-rejoining DNA breaks
Nature, 1977
Fractionation of DNA from mammalian cells by alkaline elution
Biochemistry, 1976
Fast Neutron and X-Ray Induced Single Strand DNA Breaks in Cultured Mammalian Cells
Radiology, 1976
Chromose aberrations in human lymphocytes after irradiation with 15.0-MeV neutrons in vitro I. Dose-response relation and RBE
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1975
Polyoma transformation of hamster cell clones—an investigation of genetic factors affecting cell competence
Virology, 1962