Selective purification of reduced oligosaccharides using a phenylboronic acid bond elut column: Potential application in HPLC, mass spectrometry, reductive amination procedures and antigenic/serum analysis
- 1 November 1987
- journal article
- research article
- Published by Wiley in Biomedical Chromatography
- Vol. 2 (6) , 249-253
- https://doi.org/10.1002/bmc.1130020605
Abstract
A phenylboronic acid (PBA) derivatized Bond Elut column has been investigated for the selective purification of oligosaccharide alditols and of oligosaccharide derivatives formed by reductive amination reactions. These compounds are specifically retained by the column via interaction of sterically unhindered vicinal hydroxyl groups, hence achieving their separation from glycopeptides, unreduced oligosaccharides and non-carbohydrate reactants. This purification procedure has widespread application for the clean-up of derivatives prior to HPLC, mass spectrometry and antigenic/serum analysis.Keywords
This publication has 10 references indexed in Scilit:
- Neoglycolipid micro-immunoassays applied to the oligosaccharides of human milk galactosyltransferase detect blood-group related antigens on both O- and N-linked chainsCarbohydrate Research, 1987
- Novel approach to the study of the antigenicities and receptor functions of carbohydrate chains of glycoproteinsBiochemical and Biophysical Research Communications, 1985
- Structural analysis of the O‐glycosidically linked core‐region oligosaccharides of human meconium glycoproteins which express oncofoetal antigensEuropean Journal of Biochemistry, 1985
- Structural characterization of oligosaccharides by high-performance liquid chromatography, fast-atom bombardment-mass spectrometry, and exoglycosidase digestionAnalytical Biochemistry, 1984
- The effect of mild alkali and alkaline borohydride on the carbohydrate and peptide moieties of fetuinBiochemical Society Transactions, 1984
- Reexamination of the Pyridylamination Used for Fluorescence Labeling of Oligosaccharides and Its Application to Glycoproteins 1The Journal of Biochemistry, 1984
- Evaluation of mannitol for use as a probe marker of gastrointestinal permeability in manEuropean Journal of Clinical Investigation, 1982
- Simple and fast solvent extraction system for selective and quantitative isolation of adrenaline, noradrenaline and dopamine from plasma and urineJournal of Chromatography B: Biomedical Sciences and Applications, 1982
- Continuous-flow fluorometry of low galactose concentrations in blood or plasma.Clinical Chemistry, 1980
- The Synthesis of a Di-N-acetylchitobiose Asparagine Derivative, 2-Acetamido-4-O-(2-acetamido-2-deoxy-β-d-glucopyranosyl)-1-N-(4-l-aspartyl)-2-deoxy-β-d-glucopyranosylaminePublished by Elsevier ,1970