Hydride Transfer versus Hydrogen Radical Transfer in Thymidylate Synthase
- 11 April 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 128 (17) , 5636-5637
- https://doi.org/10.1021/ja060196o
Abstract
The nature of a H-transfer in the thymidylate synthase catalyzed reaction was investigated by comparison of the wild-type enzyme with the W80M mutant. The nature of the H-transfer was not affected, as indicated by intrinsic isotope effects and their temperature dependence. These findings support a single-step hydride transfer instead of a two-step radical transfer.Keywords
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