Chromosome Localization of Highly Repetitive Human DNA's and Amplified Ribosomal DNA with Restriction Enzymes
- 28 January 1983
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 219 (4583) , 395-397
- https://doi.org/10.1126/science.6294832
Abstract
Restriction endonucleases cut and partially removed DNA throughout fixed air-dried human metaphase chromosomes. Some enzymes produced a G-banding pattern; some revealed the presence of multiple chromosome-specific classes of highly repetitive DNA in C-band heterochromatin. Enzymes that produced the informative C-band patterns had recognition sequences that were four or five, but not six, base pairs long and did not contain a cytosine-guanine doublet. In both rat and human chromosomes, regions containing amplified ribosomal RNA genes were specifically removed by the restriction endonuclease Msp I.This publication has 19 references indexed in Scilit:
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