Expression of IgD by murine lymphocytes. Loss of surface IgD indicates maturation of memory B cells.

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Abstract
B [bone marrow derived] lymphocytes capable of generating primary Ig[immunoglobulin]M and IgG plaque-forming cells (PFC) responses to burro erythrocytes have surface IgD, as do primary IgM PFC. IgG memory cells arising after 1 injection of antigen are divided into 2 groups, 1 of which expresses surface IgD while the other has no detectable membrane IgD. PFC generated from the IgG memory cells lacking surface IgD show a higher average avidity than those arising from IgD-positive IgG memory cells, indicating that mature IgG memory cells do not have surface IgD. After more than 1 injection of antigen, few, if any, IgG memory cells have surface IgD. IgG PFC arising in primary or secondary immune response lack membrane-bound IgD. These data provide the outlines for a B-cell maturation pathway in which IgD marks unprimed and early memory B cells and is lost in mature memory cells. IgD positive and IgD negative cells were isolated by the fluorescence-activated cell sorter and then assayed for activity in adoptive transfer experiments.

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