Herpesvirus vector gene transfer and expression of β–glucuronidase in the central nervous system of MRS VII mice
- 1 August 1992
- journal article
- Published by Springer Nature in Nature Genetics
- Vol. 1 (5) , 379-384
- https://doi.org/10.1038/ng0892-379
Abstract
Genetic disorders affecting the central nervous system (CNS) can potentially be treated by gene transfer using vectors which infect and express genes in post-mitotic neurons. Herpesviruses establish latent infections in neurons during which only one viral gene (LAT) is expressed, thus the LAT promoter may express foreign genes in latently infected CNS cells. Expression of a beta-glucuronidase gene driven by the LAT promoter was tested in mice lacking this enzyme, which are a model for a human genetic disease affecting the CNS (mucopolysaccharidosis VII, Sly disease). Cells expressing the missing enzymatic activity were present in the trigeminal ganglia and brainstems of latently infected animals, up to four months post-inoculation, demonstrating the potential of this approach for the long-term expression of foreign genes in the CNS.Keywords
This publication has 28 references indexed in Scilit:
- A review of the molecular mechanism of HSV-1 latencyCurrent Eye Research, 1991
- A latent, nonpathogenic HSV-1-derived vector stably expresses β-galactosidase in mouse neuronsNeuron, 1990
- β-Glucuronidase mediated pathway essential for retinal pigment epithelial degradation of glycosaminoglycans. Disease expression and in vitro disease correction using retroviral mediated cDNA transferExperimental Eye Research, 1990
- A herpes simplex virus type 1 variant, deleted in the promoter region of the latency-associated transcripts, does not produce any detectable minor RNA species during latency in the mouse trigeminal ganglionJournal of General Virology, 1990
- The DNA Sequences of the Long Repeat Region and Adjoining Parts of the Long Unique Region in the Genome of Herpes Simplex Virus Type 1Journal of General Virology, 1988
- RNA Complementary to a Herpesvirus α Gene mRNA Is Prominent in Latently Infected NeuronsScience, 1987
- Direct transfer of a lysosomal enzyme from lymphoid cells to deficient fibroblastsNature, 1981
- The structure and isomerization of herpes simplex virus genomesCell, 1979
- Beta glucuronidase deficiency: Report of clinical, radiologic, and biochemical features of a new mucopolysaccharidosisThe Journal of Pediatrics, 1973
- Dual Localization of β-Glucuronidase in Endoplasmic Reticulum and in LysosomesNature, 1967